Journal: British Journal of Nutrition

Article Title: dl-Methionine supplementation in a low-fishmeal diet affects the TOR/S6K pathway by stimulating ASCT2 amino acid transporter and insulin-like growth factor-I in the dorsal muscle of juvenile cobia (Rachycentron canadum)

doi: 10.1017/s0007114519001648

Figure Lengend Snippet: Fig. 2. Total and phosphorylation levels of target of rapamycin (TOR), S6 kinase (S6K) and 4E-binding protein 1 (4E-BP1) were examined by Western blot (A) and quantified (B–D) in the dorsal muscle of cobia fed the 0·72, 1·24, and 1·86 % methionine (Met) diets. Results are represented as means with standard errors (n 3) and were analysed using ANOVA followed by Duncan’s multiple range test. a,b Mean values with unlike letters are significantly different (P < 0·05). GAPDH, glycer- aldehyde-3-phosphate dehydrogenase.

Article Snippet: Appropriate primary antibodies were purchased from Cell Signaling Technology Inc.: phosphor-TOR (Ser2448; rabbit no. 2971), TOR (rabbit no. 2972), phospho-p70-S6K (Ser371; rabbit no. 9208), p70-S6K (rabbit no. 9202), phospho4E-BP1 (Thr37/46; rabbit no. 9459) and 4E-BP1 (rabbit no. 9452).

Techniques: Phospho-proteomics, Binding Assay, Western Blot

Journal: International immunopharmacology

Article Title: S100A8 knockdown activates the PI3K/AKT signaling pathway to inhibit microglial autophagy and improve cognitive impairment mediated by chronic sleep deprivation.

doi: 10.1016/j.intimp.2024.113375

Figure Lengend Snippet: Fig. 6. S100A8 knockdown inhibits excessive autophagy in the hippocampus of CSD mice A: Autophagosomes in the hippocampus examined by transmission electron microscopy microscopy. The autophagosomes are denoted by arrows. Scale bar: 1 µm. B-E: An Immunohistochemical staining assay was performed to detect the LC3B, P62 and Beclin-1 levels in hippocampal tissues. Scale bars: 200 and 20 µm F: Detection of S100A8 and apoptosis-related proteins in the hippocampal tissues of mice in each group by Western blot analysis. G: Western blot analysis of the protein expression of phosphorylated PI3K, Akt and P70S6K in hippocampal tissue H- N: Analysis of the protein band gray intensity. n = 5–6 mice per group; *P < 0.05 vs Control; #P < 0.05 vs CSD; &P < 0.05 vs Si-NC + CSD; $P < 0.05 vs Si-S100A8 + CSD.

Article Snippet: Antibodies against GFAP (#3670), LC3B (#83506), phosphophosphoinositide 3-kinase (PI3K) p85 (#4228), phospho-Akt (#13038), PI3K p85 (#4292), Akt (#4685), phospho-P70S6K (#97596), and P70S6K (#9202) were obtained from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Knockdown, Transmission Assay, Electron Microscopy, Microscopy, Immunohistochemical staining, Staining, Western Blot, Expressing, Control

Journal: Human pathology

Article Title: Comprehensive phenotypic and genomic characterization of venous malformations.

doi: 10.1016/j.humpath.2024.02.004

Figure Lengend Snippet: Fig. 3. Immunohistochemical analyses of the expression of mTOR pathway components in venous malformations A. Representative immunohistochemical staining patterns of phosphorylated AKT (p-AKT), p-mTOR, p-4EBP1, and p-S6K1. B. The distribution of the expression levels of these mTOR pathway components in relation to mutation types. C. Representative immunohistochemical staining patterns of mTOR pathway components in normal vessels. P-values were determined by Steel–Dwass’s multiple comparison test. mTOR; mammalian target of rapamycin, 4EBP1; eukaryotic translation initiation factor 4E-binding protein 1, S6K1; ribo somal protein S6 kinase 1.

Article Snippet: Primary antibodies against, αSMA (clone 1A4, 1:500; Dako, Denmark), phosphorylated AKT (p-AKT) (#4060, 1:100; Cell Signaling Technology, Danvers, MA, USA), p-mTOR (clone 49F9, 1:100; Cell Signaling Technology), p-4EBP1 (clone 236B4, 1:500; Cell Signaling Technology), p-S6K1 (#9204, 1:100; Cell Signaling Technology), and SP1 (#84386, 1:1600; Cell Signaling Technology) were used.

Techniques: Immunohistochemical staining, Expressing, Staining, Mutagenesis, Comparison, Binding Assay